| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002652 |
| Chromosome: | chromosome 2 |
| Location: | 2939703 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095250 | (1 of 1) 6.6.1.2 - Cobaltochelatase / Hydrogenobyrinic acid a,c-diamide cobaltochelatase | CDS | |
| Cre02.g800209 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCTGCCGTCTGCATCCGAGGAGCCGGCGTCCAATGGAGCCGTAGCG |
| Internal bar code: | TAATCAAATGGTGGTGCCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3654 |
| LEAP-Seq percent confirming: | 94.0299 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGAATCTCACGCTCAGC |
| Suggested primer 2: | GATGATGTTTGAGGTGCGGC |