| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.002690 |
| Chromosome: | chromosome 6 |
| Location: | 3248795 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277100 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTGGGATGAGCCGTACAGACCCCGGAAGGCTGCTAGCAGCTTAGCA |
| Internal bar code: | ATTGAGAGGGGTAACTATCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2352 |
| LEAP-Seq percent confirming: | 93.6508 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTCGCACAGTCGATCCA |
| Suggested primer 2: | TGTTTCCCTCACCGGTGATG |