Insertion junction: CLIP2.002702_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.002702
Chromosome:chromosome 7
Location:246297
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g313800 CGL138 Conserved in the Green Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTTTACTTTGCTTTCCGGTGCCTTGGTTGGCCTGGCCTCCGCACAGGA
Internal bar code:TTATGTGATAAAAAAAACCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2544
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCGCTAACCCATCCTGC
Suggested primer 2:CGTGGTGAGGGATGTTGTCA