| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.002702 |
| Chromosome: | chromosome 7 |
| Location: | 246305 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313800 | CGL138 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGATCAACAGGTGGTGGGCGGGAAATGAGGTGCGCGGGGGTAGGCCAA |
| Internal bar code: | CAGAGTTTTGTAGGGCTGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1703 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTGAGGGATGTTGTCA |
| Suggested primer 2: | ACTTCGCTAACCCATCCTGC |