Insertion junction: CLIP2.002704_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002704
Chromosome:chromosome 4
Location:1675464
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g212500 CGL42 (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995); Conserved in the Green Lineage 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCTGCAACATAGCTGGTGTGTGCTGCGGATGATATGTCGACTGGGTAT
Internal bar code:CGTCGTCGGCTCATGGGAGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1062
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTACCAAACGCAAGGTG
Suggested primer 2:CGTCTCGCGTGTGTTTGTTT