Insertion junction: CLIP2.002716_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002716
Chromosome:chromosome 16
Location:6446811
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g675100 CPLD53 Conserved in the Plant Lineage and Diatoms; (1 of 3) PTHR31319:SF10 - ZINC FINGER PROTEIN CONSTANS-RELATED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAAACTATTGGATGCTTTACTGTGCATCAAGTTTTATTGTTAGCAGTG
Internal bar code:GGGAATGCGGGACGTGTATAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:412
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGATTCGTGGTCCACAGTGT
Suggested primer 2:GCTCGCAGTAGTAGCCTCTG