Insertion junction: CLIP2.002719_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002719
Chromosome:chromosome 12
Location:7762532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g554250 LPB1 (1 of 1) PTHR11952:SF8 - UDP-GLUCOSE PYROPHOSPHORYLASE 3; Low Photochemical Bleaching protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCCTCGCAGAGGACATCGCACAGCAGCAGCAGCAGCAGCAGCAGGCC
Internal bar code:TTTATTCACAGACCGACGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3064
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAGTACACAGGGCAGGAC
Suggested primer 2:AAAGTAAACCGAGGAGGGCG