| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002725 |
| Chromosome: | chromosome 7 |
| Location: | 3663289 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337516 | CGL75,SMM27 | (1 of 1) PTHR18895//PTHR18895:SF74 - METHYLTRANSFERASE // HEMK METHYLTRANSFERASE FAMILY MEMBER 1; Conserved in the green lineage 75 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGGCGGCCTGGCCCGTGCTGGTGCTGGTGCCGGAGCTGGTGGCGCT |
| Internal bar code: | CCTGAATTTATAACACCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAAGAAGAACAAGGTGG |
| Suggested primer 2: | TTACCTCGAACGCGACTAGC |