| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.002725 |
| Chromosome: | chromosome 7 |
| Location: | 3663298 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337516 | CGL75,SMM27 | (1 of 1) PTHR18895//PTHR18895:SF74 - METHYLTRANSFERASE // HEMK METHYLTRANSFERASE FAMILY MEMBER 1; Conserved in the green lineage 75 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCCGCGCCACGTGCGTGTGGTGTTGGGCAGCTGGTTCGCCCCGCTGC |
| Internal bar code: | CCTGAATTTATAACACCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCTCGAACGCGACTAGC |
| Suggested primer 2: | GCGCAAGAAGAACAAGGTGG |