Insertion junction: CLIP2.002727_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002727
Chromosome:chromosome 6
Location:6691193
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g295150 (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCCTTGTGCTCTCCTCACACAACACACAGGTACGCCACGCTGGCGTT
Internal bar code:GGGTCGGTCCTGTGCGAAGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2275
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CATCACGCTGCCATTCACAG
Suggested primer 2:TTCATCGCGGTGAGTCTAGC