Insertion junction: CLIP2.002731_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002731
Chromosome:chromosome 6
Location:3401621
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278094 ELG14 Exostosin-like glycosyltransferase 14; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACCGCCGGCTCCGTGCGCAGCACATCCCCTTTCCCCCGGTGGTGGTC
Internal bar code:TTGACGCATGAATACGTCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1274
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACTTTCGAGCGGTAACT
Suggested primer 2:GGACACTACACACGCCTCAA