Insertion junction: CLIP2.002749_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.002749
Chromosome:chromosome 10
Location:2490860
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g435850 CPLD24 conserved expressed protein, perhaps chloroplast targetted; (1 of 23) IPR008927 - 6-phosphogluconate dehydrogenase C-terminal domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACATACATCGTGCCGCAAGCATACAGTTTCTAGGACATGGGTGCACTCT
Internal bar code:TTGTCGTTCGCTTGAGGGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1483
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGCTGTCGTATCGCACAA
Suggested primer 2:TCACAAGGCATTGGCTCGAT