Insertion junction: CLIP2.002789_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002789
Chromosome:chromosome 6
Location:2025167
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g265550 CDPK12 (1 of 1) PF07714//PF13202 - Protein tyrosine kinase (Pkinase_Tyr) // EF hand (EF-hand_5); Calcium/calmodulin-dependent protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGAAGGTTCCAGCGCCGATAGCCCGACCTAGCGTGTACTTCTGGTCAA
Internal bar code:TGGATACACCTAGGTACGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1912
LEAP-Seq percent confirming:38.8889
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:TACGTTCGGCTCCAGTCCTA
Suggested primer 2:TGCCCAGGCTACATCCTAGT