Insertion junction: CLIP2.002805_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002805
Chromosome:chromosome 3
Location:548516
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145207 CPLD33 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCGACCAAGGGGACGGTCCTGTACGACGTCTTCGAGTGGCAGTCGT
Internal bar code:GGGCTAGGGTTGGGCGGGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:579
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCAATGCCATCCCACAG
Suggested primer 2:GTAGCACTCGAGGATCCAGC