Insertion junction: CLIP2.002805_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002805
Chromosome:chromosome 3
Location:548520
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145207 CPLD33 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACTTGGTGGACGAGCGGGGCGCGAAGGTGCCGGCAATCTGGCGCGTG
Internal bar code:AGCATTCGGTAACTGTATAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:552
LEAP-Seq percent confirming:38.4615
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCACTCGAGGATCCAGC
Suggested primer 2:ACCTCAATGCCATCCCACAG