| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.002810 |
| Chromosome: | chromosome 12 |
| Location: | 8587627 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548651 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTCCCCAGGAGGTTTGGAAGCGGCTAGCACAAGCAGCTCGATCTCCC |
| Internal bar code: | TAGCTGGTTCACAGATGAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2309 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATGTGAAGGCCAAACG |
| Suggested primer 2: | ACGTTGTCGGCTTCTACGTT |