| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.002812 |
| Chromosome: | chromosome 5 |
| Location: | 769417 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245701 | FAP104 | Flagellar Associated Protein 104 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGCCCCTCCCCGCACTCCCAGAGCCCTTCCCTCCTCACAATTCCCAA |
| Internal bar code: | TTTACTGGTCGGGAAGGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 52.6316 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGAGAGGGGAGAGAGGC |
| Suggested primer 2: | CAATGGCACTCACCGCAAAA |