Insertion junction: CLIP2.002829_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.002829
Chromosome:chromosome 12
Location:1998422
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g510850 CGL73 expressed protein Conserved in the Green Lineage; (1 of 2) PF11833 - Protein of unknown function (DUF3353) (DUF3353) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGGGTCACGCTCCCCAAGCCGAGGGAACGGCTTGTAGTCTCCATCTG
Internal bar code:CGCGTCTACACTTGACTACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2782
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGTCACAGGTGCAACGAC
Suggested primer 2:AGAGCGACAAAGTGACAGGG