| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.002830 |
| Chromosome: | chromosome 10 |
| Location: | 3283247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442950 | (1 of 2) PF13483 - Beta-lactamase superfamily domain (Lactamase_B_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTTGATGACTGCCGTGTTAACCTGCACGATATGCAGATTGCATCAGT |
| Internal bar code: | TATCTTCAGATATTAAGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2186 |
| LEAP-Seq percent confirming: | 57.8947 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTACAGGTGCTTCCCCT |
| Suggested primer 2: | AGACCTCCTCCATCTCCACC |