Insertion junction: CLIP2.002848_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002848
Chromosome:chromosome 2
Location:3370428
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g098200 CGL103,PRT1 (1 of 1) PF00569//PF13920 - Zinc finger, ZZ type (ZZ) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3); Conserved in the Green Lineage 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTATAACCAGGTAGACAGATTGGTTTTGCGCTTCTCCCCAACCGCGCGT
Internal bar code:TAGCGGTGACTAAGCTGCATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1154
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACAGGACCATGTTCACG
Suggested primer 2:AAATAGCCGCCCTATCAGCC