Insertion junction: CLIP2.002851_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002851
Chromosome:chromosome 16
Location:5374530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAAAGCTGCACTACTGCACCACCGAAGCTCCGGGTGGCCTCCCGCAC
Internal bar code:CCTAGTGGGTCCTTGTCTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3603
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:130

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCGCAAATGATCCTCG
Suggested primer 2:CAAAGGGAGAGAAGCAGGGG