| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002858 |
| Chromosome: | chromosome 7 |
| Location: | 5813624 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353500 | CGLD5A | ethylene response element DNA-binding domain containing protein; (1 of 1) PTHR31190:SF30 - ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 15-RELATED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGAGGTAGGCCGCTTACATACTCGTGGTGGTGCGTGTGTGGCCTCCAG |
| Internal bar code: | ATGCGACGTAAACTTCCTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2601 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTTGCTGTTGTTGCT |
| Suggested primer 2: | GCGACTGCGTAATTCGTGTC |