Insertion junction: CLIP2.002858_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002858
Chromosome:chromosome 7
Location:5813625
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353500 CGLD5A ethylene response element DNA-binding domain containing protein; (1 of 1) PTHR31190:SF30 - ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 15-RELATED 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTTCCTCACACGATTCGAGAGAGACTACTGCATGACGCCACGGGTGGG
Internal bar code:GCTCGCTATACGAATGGCTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:759
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGACTGCGTAATTCGTGTC
Suggested primer 2:CTGCTGTTGCTGTTGTTGCT