| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002867 |
| Chromosome: | chromosome 5 |
| Location: | 911087 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246551 | (1 of 1) IPR000104//IPR001810//IPR020683 - Antifreeze protein, type I // F-box domain // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGTTCTGGCCAGGGGAGGGCCAGGGGCTGCAGGGGAGGGCCAGGGG |
| Internal bar code: | CCGCGCGGATACAGTTGGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1743 |
| LEAP-Seq percent confirming: | 91.5254 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATGTAGCTCCCGCATGT |
| Suggested primer 2: | TTGCTTGCCGGGTTGAATTG |