Insertion junction: CLIP2.002878_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.002878
Chromosome:chromosome 9
Location:5378758
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g408051 (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTTAAATGTGGCACATCGCTGTCGAATACCTCACTACTGGGTGTTAG
Internal bar code:TTACATTCGGTTGTTGTACTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:182
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCGCTCATAGTCATCCGG
Suggested primer 2:GGGTGGGTGAGGTGTGTATG