Insertion junction: CLIP2.002882_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.002882
Chromosome:chromosome 6
Location:1425434
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259900 ATPC1,ATPC Chloroplast ATP synthase gamma chain; (1 of 1) K02115 - F-type H+-transporting ATPase subunit gamma (ATPF1G, atpG) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCATCAGATCCTGAACGCTCACGTCGAGTTCTATTCATTTGGACCATG
Internal bar code:CATATATATACTACACGAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:5167
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:123
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:123

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGAATTTGCGTGGTTGGT
Suggested primer 2:TTTTCTTGCGTGAGTGCGTG