| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.002887 |
| Chromosome: | chromosome 14 |
| Location: | 2292276 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622550 | (1 of 1) PTHR10953//PTHR10953:SF145 - UBIQUITIN-ACTIVATING ENZYME E1 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACACACACACACACGCACCTGCGCCCATGCTAGAGAT |
| Internal bar code: | CGAATGAGTGAGCGTTGTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1319 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTCTAATAGTGCCCTCC |
| Suggested primer 2: | GGCGAAGGAGTCTCCATACG |