Insertion junction: CLIP2.002894_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002894
Chromosome:chromosome 3
Location:6656734
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g195200 HLD1,CGL76 Conserved in the Green Lineage; (1 of 6) IPR000073//IPR000639//IPR029058 - Alpha/beta hydrolase fold-1 // Epoxide hydrolase-like // Alpha/Beta hydrolase fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGACGCAGCCTCAATCAGGGATTGTCTTCTCCGGCACATTGCTGAA
Internal bar code:TGTTTGTAGTAGGGATCGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1492
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGGAATCACAAGGGGTC
Suggested primer 2:CAACCTGACTGTCCCCTTCC