| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002895 |
| Chromosome: | chromosome 7 |
| Location: | 335225 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314450 | SMM22,CGL33B | (1 of 1) PTHR12176:SF19 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; conserved methyltransferase of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGTCTGAATGACGAAGGGAGGATCAGGCGCTGTGGCAGTCATGGAC |
| Internal bar code: | CGAGCGTGCGGCTGGGGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2862 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATGGACTCCCACGTCTC |
| Suggested primer 2: | TTACTGCGTGTGAGCATGGT |