| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.002906 |
| Chromosome: | chromosome 6 |
| Location: | 2351804 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267850 | PLS1 | Phospholipid scramblase; (1 of 1) IPR005552//IPR025659 - Scramblase // Tubby C-terminal-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCACAGGCGGTGAAGCACGTTGATGGCCTTGCAGCGGGTGGGGTGGGC |
| Internal bar code: | GCTCAGTCAGGCCGCTGATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1452 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTTGAGCTCGAACTCCC |
| Suggested primer 2: | ATCAGACTCGGACTCTGCCT |