| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.002908 |
| Chromosome: | chromosome 10 |
| Location: | 2817597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438883 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGCCACCGCCCGATCTGACGGTGACGCTGAGGTCCTCCAGGTGCTGC |
| Internal bar code: | GGGGGTGTATTAGCCGGAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2580 |
| LEAP-Seq percent confirming: | 87.7193 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGTCATAATAGGCGGGG |
| Suggested primer 2: | ATTGACATGACCTGCCGTGT |