Insertion junction: CLIP2.002924_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.002924
Chromosome:chromosome 2
Location:586980
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g077150 CPLD14 Conserved in the Plant Lineage and Diatoms; (1 of 2) PF05684 - Protein of unknown function (DUF819) (DUF819) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCATCGGTGCCCCTCGGCGCATCCCCACCCACGCCCACGTTTGCAGA
Internal bar code:TCTTGAGGCACATATCTATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAGCAAGACAGCTGGACC
Suggested primer 2:CAACCGACTGTAACGACCCA