Insertion junction: CLIP2.002977_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.002977
Chromosome:chromosome 17
Location:1171258
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g704350 GLOD4 (1 of 1) PTHR10374//PTHR10374:SF20 - LACTOYLGLUTATHIONE LYASE GLYOXALASE I // GLYOXALASE DOMAIN-CONTAINING PROTEIN 4; Glyoxalase domain containing 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTGCCCGGGGTGTCCAGAGACACGTACGGCGTGTGCACCGTGTAGCCG
Internal bar code:CTACGTTCCCGCTGCGGCTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3560
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGGTAAGTGGCGGAACT
Suggested primer 2:CCTCCCATCAACCTTGCCAT