Insertion junction: CLIP2.002985_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.002985
Chromosome:chromosome 1
Location:5310530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g037350 TEH2,CGLD2 Thioesterase family protein; (1 of 2) K17361 - acyl-coenzyme A thioesterase 9 [EC:3.1.2.-] (ACOT9) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCTTTGACGCGCATGCAACCGATGTATAAACTGCAACCACCAAATAC
Internal bar code:GTACTCTGTGTTTATGGCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1171
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGCTTCTTGGTGATGGGG
Suggested primer 2:CGTTGTGGGTTCGTTTGGAC