| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.002998 |
| Chromosome: | chromosome 7 |
| Location: | 3693773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337650 | PDC1 | Mitochondrial pyruvate dehydrogenase complex, E1 component, alpha subunit; (1 of 1) PTHR11516//PTHR11516:SF40 - PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR // PDHA1 PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGAAACTAAATGCGTGCTTCAAAGTACGCTTCAAAGCCAGGGATGGCT |
| Internal bar code: | AGATCGGGATGGGCTCAAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1655 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCCACGTGTCAGCAAT |
| Suggested primer 2: | CTGTCAAACAGCCCCCATCT |