Insertion junction: CLIP2.003008_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003008
Chromosome:chromosome 14
Location:1949789
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g620600 PHC2,FAP202 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTCAAAGATAATCATTTACGCTCACGGCGTAAATAGTGGTCGTGTGGG
Internal bar code:ACAGTCATTCTATATTGTAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:936
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCACCCAGCTTGTAGGC
Suggested primer 2:ACACGCATCTGAGACTGCAA