| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003015 |
| Chromosome: | chromosome 11 |
| Location: | 1518910 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468750 | CPLD48 | Conserved in the Plant Lineage and Diatoms; (1 of 3) PF07386 - Protein of unknown function (DUF1499) (DUF1499) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACCCAGGTTGCCGGGGCGAGGGGCCGCGCCGATGGACAGCAGCGCGCC |
| Internal bar code: | AGACAGACCACCCTTTCAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1417 |
| LEAP-Seq percent confirming: | 9.80392 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCTGGTATCGAGCTCGG |
| Suggested primer 2: | TGTGCGTGTCAGGTCAAGTT |