Insertion junction: CLIP2.003021_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003021
Chromosome:chromosome 12
Location:4493471
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g520600 PRPS6 (1 of 1) PTHR21011//PTHR21011:SF1 - MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6 // 28S RIBOSOMAL PROTEIN S6, MITOCHONDRIAL; Chloroplast Ribosomal Protein S6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGCCGACGCCGCCATGTGGCGGCTGCTTATACCTTCTTGTTCCCCCCT
Internal bar code:ACATGGTATCGGACTTCTTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4981
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:122

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCACCCCATCTGCATCTA
Suggested primer 2:GGCCTCCTCTGAGCGAAAAT