Insertion junction: CLIP2.003025_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003025
Chromosome:chromosome 16
Location:6239739
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g676450 CPLD39 Conserved in the Plant Lineage and Diatoms 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGAGGTTGTCTGTTGCGCGGACTCTCTTTGGGGTTGCAACTGCAGTGG
Internal bar code:AATAGTCCACGAGCACCCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1659
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGGCAAGTATGAATGCG
Suggested primer 2:TACTGCTCGAAACCTGCCAG