| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.003057 |
| Chromosome: | chromosome 10 |
| Location: | 6423219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464600 | CAM14,EZY15 | (1 of 1) IPR002048//IPR006311 - EF-hand domain // Twin-arginine translocation pathway, signal sequence; Calmodulin-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTACACCAAGATTTTGGAGGGCAGCTGCAGCTGGGAGACCGCGCAAAC |
| Internal bar code: | TGCTCTTTTGGCGTTATCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2921 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATATTGCCGACACAACC |
| Suggested primer 2: | CTTGTCGGGCATGCATCATG |