Insertion junction: CLIP2.003062_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.003062
Chromosome:chromosome 13
Location:1912044
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g575900 CGL117 (1 of 1) PF04959//PF12066 - Arsenite-resistance protein 2 (ARS2) // Domain of unknown function (DUF3546) (DUF3546); predicted protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGTGAATTTGTCTGAGATGATATAGCACTTATGAACAAATCATTTATC
Internal bar code:TTGCGATTAATCTTGATCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2214
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGCTAATGCCTAAACGC
Suggested primer 2:TTGCTGCACTCACTAGGGTG