| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003076 |
| Chromosome: | chromosome 4 |
| Location: | 3930293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231026 | SRP43,cpSRP43 | (1 of 1) K12271 - signal recognition particle 43 kDa protein (SRP43, CAO); 43 kDa subunit of chloroplast signal recognition particle | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATGGGACGGGAAGAGCGGGCGGGAGGGGGAGCGTAAGCTGCGTGCAG |
| Internal bar code: | CGGCGTGCGGTACACCGATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1890 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCTTCGCTGAAGGCCCTC |
| Suggested primer 2: | GGTTGTGCTGACGGTGTCTA |