Insertion junction: CLIP2.003087_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.003087
Chromosome:chromosome 7
Location:5159531
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g348550 TGL13 (1 of 3) PTHR21493:SF153 - PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGTGTGCCGTGTCATTGTATTAGGGAAGCACAAATGGGGAAGGGGCC
Internal bar code:CAATGAAAATAATCTCTCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1572
LEAP-Seq percent confirming:97.2222
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAAACACGTCCCACAGGC
Suggested primer 2:AGTGGGTTTTGTTGGCCAGA