Insertion junction: CLIP2.003112_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.003112
Chromosome:chromosome 6
Location:3513811
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278117 CPLD7 Conserved in the Plant Lineage and Diatoms; (1 of 98) IPR023214 - HAD-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGTCGGACAGGGAGGGGTCCCTGCGCACGTGTGGAAGGGTGAAGACA
Internal bar code:TTCAGGTTTGGCTGCGCCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTTCCGACTCCTGCTCCT
Suggested primer 2:TACCATCAGCCTCATCCCCA