| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003116 |
| Chromosome: | chromosome 10 |
| Location: | 4073391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448051 | SFL1,CPSFL1 | (1 of 1) PTHR10174:SF156 - SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN; CRAL-TRIO hydrophobic ligand-binding (Sec14) domain protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCCGGGCGGCGAGCAGATTGTTGGCATCTTCGACCTGCGCGGCTT |
| Internal bar code: | AGTTTACCACGGGTAATAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2914 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCTAGAATCCGTCCCAG |
| Suggested primer 2: | ACACGCTCAAGTGGTACCTG |