Insertion junction: CLIP2.003140_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.003140
Chromosome:chromosome 10
Location:26059
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g417550 CLPS3,CLPT3 (1 of 2) 3.6.4.10 - Non-chaperonin molecular chaperone ATPase / Molecular chaperone Hsc70 ATPase; Additional subunit of chloroplast ClpP complex 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGCAGTCTGTCTTGGGACCTCTTTATATGCAACTAATACGAATTATG
Internal bar code:AATGTGCGGTTGAGACGTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1459
LEAP-Seq percent confirming:72.2222
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCGTGAAGGTTCCAGAA
Suggested primer 2:GACCTACAGTGAACAGGCCC