Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.003154 |
Chromosome: | chromosome 8 |
Location: | 1627482 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g365800 | (1 of 1) IPR000104//IPR001077//IPR016461//IPR029063 - Antifreeze protein, type I // O-methyltransferase, family 2 // O-methyltransferase COMT-type // S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAGTGTTGGTGTCTTAGGTACTATATATGTCACGCTACATATGGACG |
Internal bar code: | TTCCGGATAGCTGTGAGGGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 561 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 16 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAATGCTCCAGGTCAGTCG |
Suggested primer 2: | CCCGAGTGTGTGTGCAGTAT |