| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003165 |
| Chromosome: | chromosome 7 |
| Location: | 5559502 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351825 | (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGTGTGGTTCAGTCGCCGGATCCGCGAAACTTGCAGGACGGCTGGGT |
| Internal bar code: | CTTCATACATGAGAGTTCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4074 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGCAGACAGTGGAGAA |
| Suggested primer 2: | GCTGTGACCAACTGAGAGCT |