| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003175 |
| Chromosome: | chromosome 10 |
| Location: | 4686640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452100 | PSBY,PSBY2,PSBY1 | (1 of 1) K02723 - photosystem II PsbY protein (psbY); ycf32-related polyprotein of photosystem II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCCCATGAGCCTAGGAGTGCGGCTTCAGTGTGTACCGTTACTCTTGT |
| Internal bar code: | ATGGAGATTTATGTGGTGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4067 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGCTGGGAATGGTGAC |
| Suggested primer 2: | GACTTCGGGAGATGAGAGCG |