| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.003183 |
| Chromosome: | chromosome 8 |
| Location: | 3704441 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380250 | CP12 | (1 of 2) PF02672 - CP12 domain (CP12); Calvin cycle protein CP12, chloroplastic | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCGCGGACCGCTCCCTTCGCTTGCACAACGTTGCGTCTTGTTTTTCT |
| Internal bar code: | ATGAGACGAACGTTTTCGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACACCCTTGAGTCGAGA |
| Suggested primer 2: | CAGCTCACGGCATATAGGCA |